Distribution, driving forces, and risk assessment of 2-MIB and its producer in a drinking water source-oriented shallow lake.

Freshwater blooms of harmful cyanobacteria in drinking water source-oriented shallow lakes affect public health and ecosystem services worldwide. Therefore, identifying 2-methylisoborneol (2-MIB)-producing cyanobacteria and predicting the risks of 2-MIB are critical for managing 2-MIB-infected water sources. Previous studies on the potential producers and risks of 2-MIB have focused on reservoirs or have been limited by the ecosystems of phytoplankton-dominated areas. We investigated the producers, distribution, and occurrence of 2-MIB in East Taihu Lake-a drinking water source-oriented shallow lake with macrophyte- and phytoplankton-dominated areas-from August 2020 to November 2021. We observed that Pseudanabaena sp. produces 2-MIB in this lake, as determined by the maximum correlation coefficient (R = 0.71, p < 0.001), maximum detection rate, and minimum false positive/negative ratio exhibited by this genus. Extreme odor events occurred in this lake during late summer and early autumn in 2021, with the mean 2-MIB concentration increasing to 727 ± 426 ng/L and 369 ± 176 ng/L in August and September, respectively. Moreover, the macrophyte-dominated area, particularly the wetland area, exhibited a significant decrease (p < 0.01) in bloom intensity and 2-MIB production during these extreme odor events. Pseudanabaena sp. outbreak was likely owing to eutrophication, seasonal gradients, and macrophyte reduction, considering that temporal trends were consistent with high water temperature, high total phosphorus levels, and low-light conditions. Moreover, 2-MIB production was sensitive to short-term hydrometeorological processes, with high water levels and radiant intensity enhancing 2-MIB production. The risk assessment results showed that the probability of 2-MIB concentration exceeding the odor threshold (10 ng/L) is up to 90% when the cell density of Pseudanabaena sp. reaches 1.8 × 107 cell/L; this risk is reduced to 50 and 25% at densities of < 3.8 × 105 cell/L and 5.6 × 104 cell/L, respectively. Our findings support calls for shallow lake management efforts to maintain a macrophyte-dominated state and control odorous cyanobacteria growth.

Driving forces and recovery potential of the macrophyte decline in East Taihu Lake.

Macrophytes are of key importance to the structure and ecological services of shallow lakes and are sensitive to anthropogenic and natural perturbations. Ongoing eutrophication and hydrological regime change affect macrophytes through changes in water transparency and water level, which lead to a dramatic decrease in bottom light availability. Here an integrated dataset (2005-2021) of multiple environmental factors is used to demonstrate the driving forces and recovery potential of the macrophyte decline in East Taihu Lake by using a critical indicator, which is the ratio of the Secchi disk depth to the water depth (SD/WD). The macrophyte distribution area showed a remarkable decrease from 136.1 ± 9.7 km2 (2005-2014) to 66.1 ± 6.5 km2 (2015-2021). The macrophyte coverage in the lake and in the buffer zone decreased by 51.4% and 82.8%, respectively. The structural equation model and correlation analysis showed that the distribution and coverage of macrophytes decreased with the decrease in the SD/WD over time. Moreover, an extensive hydrological regime change, which caused a sharp decrease in SD and an increase in the water level, is likely to be the driving force that brought about the decline of macrophytes in this lake. The proposed recovery potential model shows that the SD/WD has been low in recent years (2015-2021), and that this SD/WD cannot ensure the growth of submerged macrophytes and is unlikely to ensure the growth of floating-leaved macrophytes, especially in the buffer zone. The approach developed in the present study provides a basis for the assessment of macrophyte recovery potential and the management of ecosystems in shallow lakes that suffer from macrophyte loss.

Impact of submerged macrophytes on growth and 2-MIB release risk of Pseudanabaena sp.: From field monitoringa to cultural experiments.

The off-flavor compound 2-methylisoborneol (2-MIB) is generally associated with the proliferation and metabolism of filamentous cyanobacteria in shallow freshwater ecosystems. Here field monitoring in East Taihu Lake from July to October 2021, along with cultural experiments, was conducted to determine the impact of submerged macrophytes on the growth and 2-MIB production of filamentous cyanobacteria. Pseudanabaena sp. was identified as the 2-MIB producer with the highest detection rate (100%) and correlation coefficient (R=0.68, p < 0.001). The 2-MIB concentration and algal growth in the macrophyte-dominated zones were markedly decreased compared with those in the phytoplankton-dominated zone. Five submerged macrophytes classified into flat-leaf type (Vallisneria natans and Potamogeton crispus) and thin-leaf type (Hydrilla verticillata, Ceratophyllum demersum, and Myriophyllum spicatum) exhibited strong inhibition effects against Pseudanabaena sp.: Overall inhibition efficiencies (IEs) of 92.7% ± 6.8% and 92.7% ± 8.4% for cell growth and 2-MIB production were achieved, respectively. Moreover, the thin-leaf macrophytes exhibited significant higher IEs for cell growth (94.0% vs. 84.7%) and 2-MIB production (99.4% vs. 82.6%) than the flat-leaf macrophytes and can be selected as pioneer species in controlling odor problems. Nutrient uptake, increasing water clarity, shading effects, and allelopathic effects of the submerged macrophytes were found to be the dominant inhibition mechanisms.

Morphological responses of the submerged macrophyte Vallisneria natans along an underwater light gradient: A mesocosm experiment reveals the importance of the Secchi depth to water depth ratio.

Bottom light availability (BLA), represented by the ratio of the Secchi disk depth to water depth (SD/WD), plays a fundamental role in the growth and reproduction of submerged macrophytes. However, studies thus far have mainly explored the interactions between macrophyte responses and BLA through field investigations; this means that knowledge of such responses to various underwater light conditions in mesocosm experiments is rudimentary at best. We hypothesized that the growth and clonal reproduction of submerged macrophytes decrease with decreasing BLA and collapse beyond a critical threshold. Here we performed a 42-day outdoor mesocosm experiment with a species of perennial submerged macrophyte, Vallisneria natans, along a decreasing SD/WD gradient. Over this gradient, the primary morphological traits (plant height, root length, plant biomass), relative growth rate, and shoot increment rate of V. natans exhibited a significant trend of initial increase followed by a decrease. The photoinhibition occurred at high and low-light stress, indicating that an intermediate SD/WD (0.55-0.65) provides optimal growth conditions. The number of ramets, ramet biomass, ramet/total biomass ratio, and root/shoot ratio all decreased with decreasing SD/WD ratio, suggesting that V. natans allocates more resources for clonal reproduction and population stability rather than increased shoot biomass at higher BLA conditions. The results of principal component analysis and threshold detection indicated that the growth traits of V. natans had a higher SD/WD tipping point value (0.55 vs. 0.50) than the reproductive capacity and stability, indicating that only values of SD/WD ≥ 0.55 ensured the growth and the vegetative reproduction of V. natans. Additionally, an inverted U-shaped relationship between growth traits and a linear relationship between reproduction and stability reflect the resource allocation strategies and resilience of V. natans to decreasing underwater light conditions.

The role of mechanical harvesting on the recession of aquatic vegetation under an extreme water level increase in a eutrophic shallow lake.

Mechanical harvesting is quick and effective way to remove nuisance macrophytes and improve recreational use and aesthetics in shallow lake. However, applying mechanical harvesting to macrophytes in eutrophic shallow lake with weak resilience and strong perturbation raises concerns of the public and scientific communities. A combination of field investigation and remote sensing was used to determine the potential driving factors of macrophyte degradation in a eutrophic shallow lake from 2014 to 2017, including a comparative analysis of preserved and harvested areas to determine the impacts of mechanical harvesting. Over 95% of macrophytes had disappeared by 2017 in both preserved and harvested areas, with no significant difference in macrophyte distribution area or decline rate between the two by that time. The decline rate in the harvested area (76.7%) was slightly higher than in the preserved area (61.7%) in 2016 after performing the mechanical harvesting in 2015. The results demonstrate that mechanical harvesting is not the definitive driving factor for macrophyte loss, but it could accelerate the decline process. Bottom light availability (Secchi disk depth to water level), which decreased from 0.70 to 0.29 in 2015 and from 0.70 to 0.21 in 2016, is more likely the driving factor, caused by extreme water level increase events in two consecutive years (2015 and 2016) and decreased water clarity. Maintaining water clarity and low water level is crucial for macrophyte restoration.

Embedded reservoir and constructed wetland for drinking water source protection: Effects on nutrient removal and phytoplankton succession.

An embedded reservoir that provides an efficient nutrient removal system protects drinking water. However, embedded reservoirs are rarely used in eutrophic shallow lakes because of their undetermined nutrient retention efficiency and unknown effects by the phytoplankton community. In this study, we aim to investigate the nutrient retention and algae succession in an embedded reservoir and adjacent wetland from April 2017 to September 2018 in the eastern part of Lake Taihu, China. More than 40% of total phosphorus (TP) and 45% of particulate phosphorous entering the reservoir were retained semi-annually, and the highest TP removal efficiency was achieved in the reservoir during autumn with an average value of 53.3% ±â€¯9.9%. The overall nitrogen retention efficiency (21.7% ±â€¯37.8%) was lower than that of TP (41.8% ±â€¯27.8%). Similar trends were obtained in the wetland area. An important pathway for phosphorus removal is through particulate matter retention. Our study revealed that nutrient retention mechanisms in the reservoir were primarily via macrophyte absorption, particulate substance sedimentation, and prolonged water residence time. Consequently, the phytoplankton biomass (Chl-a) in the reservoir decreased (from 48.0 to 25.2 µg/L) and water transparency improved, due to the decreased P level and transformation of the phytoplankton group into simple structures with good ecological status. Therefore, the combination of embedded reservoir and constructed wetland ecosystem can be used successfully to protect surface water. The results will be advantageous to groups seeking to preserve drinking water sources.